zebrafish codon optimized cas9 (Addgene inc)
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zebrafish codon optimized cas9
Zebrafish Codon Optimized Cas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 221 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/zebrafish+codon+optimized+cas9/pT3TS-nCas9n+(Plasmid+%2346757)/bio_rxiv__2025__10__24__684380-34-1-5
Average 95 stars, based on 221 article reviews
Zebrafish Codon Optimized Cas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 221 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/zebrafish+codon+optimized+cas9/pT3TS-nCas9n+(Plasmid+%2346757)/bio_rxiv__2025__10__24__684380-34-1-5
Average 95 stars, based on 221 article reviews
zebrafish codon optimized cas9 - by Bioz Stars,
2026-11
95/100 stars
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Plasmid Preparation:Article Title: Loss of zebrafish dcst2 expression is not associated with muscle abnormalities Article Snippet: .. Briefly, a Article Title: CHCHD10 P80L knock-in zebrafish display a mild ALS-like phenotype. Article Snippet: .. Briefly, a Article Title: Loss of mitochondrial Chchd10 or Chchd2 in zebrafish leads to an ALS-like phenotype and Complex I deficiency independent of the mitochondrial integrated stress response. Article Snippet: Funding information Canadian Institutes of Health Research, Grant/Award Numbers: 420108, 133530; Natural Sciences and Engineering Research Council of Canada, Grant/Award Number: RGPIN-2018-05603; ALS Canada—Brain Canada Discovery grant; Rare Diseases: Models & Mechanisms Network grant Abstract Mutations in CHCHD10 and CHCHD2, encoding two paralogous mitochondrial proteins, have been identified in cases of amyotrophic lateral sclerosis, frontotemporal lobar degeneration, and Parkinson’s disease.. Their role in disease is unclear, though both have been linked to mitochondrial respiration and mitochondrial stress responses.. Here, we investigated the biological roles of these proteins during vertebrate development using knockout (KO) models in zebrafish. Article Title: Impaired larval motor function in a zebrafish Stathmin-2 (STMN2) knockout model Article Snippet: Target gRNA sites were identified using CRISPRscan , synthesized using the T7 MEGAscript kit (Invitrogen), and purified by phenol-chloroform extraction and ethanol precipitation. .. A Article Title: NIPSNAP1 and NIPSNAP2 Act as "Eat Me" Signals for Mitophagy. Article Snippet: .. A plasmid encoding Article Title: Loss of dcst2 expression in male zebrafish is not associated with muscle hypertrophy. Article Snippet: variant) was identified in DCST2 that segregated in an autosomal dominant fashion in male and female individuals with a supranormal strength, muscle hypertrophy and myalgia in a large French-Canadian family (Brais et al. 2016).. Recently, DCST2 and its paralog DCST1, were implicated in playing a critical role in sperm fertilization of the egg in both zebrafish and mice (Inoue et al. 2021; Noda et al. 2022).. Zebrafish KO models of either dcst1 or dcst2 results in a sperm-egg binding defect resulting in male sterility (Noda et al. 2022). In Vitro:Article Title: Loss of zebrafish dcst2 expression is not associated with muscle abnormalities Article Snippet: .. Briefly, a Article Title: CHCHD10 P80L knock-in zebrafish display a mild ALS-like phenotype. Article Snippet: .. Briefly, a Article Title: Loss of mitochondrial Chchd10 or Chchd2 in zebrafish leads to an ALS-like phenotype and Complex I deficiency independent of the mitochondrial integrated stress response. Article Snippet: Funding information Canadian Institutes of Health Research, Grant/Award Numbers: 420108, 133530; Natural Sciences and Engineering Research Council of Canada, Grant/Award Number: RGPIN-2018-05603; ALS Canada—Brain Canada Discovery grant; Rare Diseases: Models & Mechanisms Network grant Abstract Mutations in CHCHD10 and CHCHD2, encoding two paralogous mitochondrial proteins, have been identified in cases of amyotrophic lateral sclerosis, frontotemporal lobar degeneration, and Parkinson’s disease.. Their role in disease is unclear, though both have been linked to mitochondrial respiration and mitochondrial stress responses.. Here, we investigated the biological roles of these proteins during vertebrate development using knockout (KO) models in zebrafish. Article Title: Impaired larval motor function in a zebrafish Stathmin-2 (STMN2) knockout model Article Snippet: Target gRNA sites were identified using CRISPRscan , synthesized using the T7 MEGAscript kit (Invitrogen), and purified by phenol-chloroform extraction and ethanol precipitation. .. A Article Title: Loss of dcst2 expression in male zebrafish is not associated with muscle hypertrophy. Article Snippet: variant) was identified in DCST2 that segregated in an autosomal dominant fashion in male and female individuals with a supranormal strength, muscle hypertrophy and myalgia in a large French-Canadian family (Brais et al. 2016).. Recently, DCST2 and its paralog DCST1, were implicated in playing a critical role in sperm fertilization of the egg in both zebrafish and mice (Inoue et al. 2021; Noda et al. 2022).. Zebrafish KO models of either dcst1 or dcst2 results in a sperm-egg binding defect resulting in male sterility (Noda et al. 2022). Extraction:Article Title: Loss of zebrafish dcst2 expression is not associated with muscle abnormalities Article Snippet: .. Briefly, a Article Title: CHCHD10 P80L knock-in zebrafish display a mild ALS-like phenotype. Article Snippet: .. Briefly, a Article Title: Loss of mitochondrial Chchd10 or Chchd2 in zebrafish leads to an ALS-like phenotype and Complex I deficiency independent of the mitochondrial integrated stress response. Article Snippet: Funding information Canadian Institutes of Health Research, Grant/Award Numbers: 420108, 133530; Natural Sciences and Engineering Research Council of Canada, Grant/Award Number: RGPIN-2018-05603; ALS Canada—Brain Canada Discovery grant; Rare Diseases: Models & Mechanisms Network grant Abstract Mutations in CHCHD10 and CHCHD2, encoding two paralogous mitochondrial proteins, have been identified in cases of amyotrophic lateral sclerosis, frontotemporal lobar degeneration, and Parkinson’s disease.. Their role in disease is unclear, though both have been linked to mitochondrial respiration and mitochondrial stress responses.. Here, we investigated the biological roles of these proteins during vertebrate development using knockout (KO) models in zebrafish. Article Title: Impaired larval motor function in a zebrafish Stathmin-2 (STMN2) knockout model Article Snippet: Target gRNA sites were identified using CRISPRscan , synthesized using the T7 MEGAscript kit (Invitrogen), and purified by phenol-chloroform extraction and ethanol precipitation. .. A Article Title: Loss of dcst2 expression in male zebrafish is not associated with muscle hypertrophy. Article Snippet: variant) was identified in DCST2 that segregated in an autosomal dominant fashion in male and female individuals with a supranormal strength, muscle hypertrophy and myalgia in a large French-Canadian family (Brais et al. 2016).. Recently, DCST2 and its paralog DCST1, were implicated in playing a critical role in sperm fertilization of the egg in both zebrafish and mice (Inoue et al. 2021; Noda et al. 2022).. Zebrafish KO models of either dcst1 or dcst2 results in a sperm-egg binding defect resulting in male sterility (Noda et al. 2022). Polymerase Chain Reaction:Article Title: TEMPO enables sequential genetic labeling and manipulation of vertebrate cell lineages. Article Snippet: .. For this, we PCR amplified a Amplification:Article Title: TEMPO enables sequential genetic labeling and manipulation of vertebrate cell lineages. Article Snippet: .. For this, we PCR amplified a Purification:Article Title: Impaired larval motor function in a zebrafish Stathmin-2 (STMN2) knockout model Article Snippet: Target gRNA sites were identified using CRISPRscan , synthesized using the T7 MEGAscript kit (Invitrogen), and purified by phenol-chloroform extraction and ethanol precipitation. .. A Ethanol Precipitation:Article Title: Impaired larval motor function in a zebrafish Stathmin-2 (STMN2) knockout model Article Snippet: Target gRNA sites were identified using CRISPRscan , synthesized using the T7 MEGAscript kit (Invitrogen), and purified by phenol-chloroform extraction and ethanol precipitation. .. A Generated:Article Title: NIPSNAP1 and NIPSNAP2 Act as "Eat Me" Signals for Mitophagy. Article Snippet: .. A plasmid encoding |